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    From Imparting Functionality to Shielding Against Unfolding via Embedding Enzymes into Metal-organic Frameworks: the insight of material biology
    Update time: 2017-08-22
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    From Imparting Functionality to Shielding Against Unfolding via Embedding Enzymes into Metal-organic Frameworks: the insight of material biology


    Fa-Kuen Shieh, associate professor, National Central University


    9:30-11:00 am, August 27, 2017, Sunday  


    216 Meeting Room, Energy Building  


    Metal-organic Frameworks (MOFs) are a crystalline porous material composed of metal nodes and organic linkers, where the physicochemical properties of MOFs can be controlled through careful selection of the inorganic or organic precursors. Therefore, MOFs show promise for a wide range of applications ranging from gas separation to biomedical science. Especially, immobilizing enzyme on solid supports is of great interest for industry application and MOFs have become a desired support material due to their versatile chemical properties. Previous study has showed their potential by post-immobilization approaches in MOFs, yet still with the problems of enzyme leaking and the selection of enzymes is restricted in the pore size of MOFs.

    Recently, our group has pioneered a de novo approach to embed enzyme in MOFs. This is very different from most of the previous post-immobilization approaches, in which the enzymes are introduced into the pre-synthesized MOF crystals. This new approach provides a new tool to immobilize enzymes and has a great advantage compared to the previous works: the MOFs of pore sizes smaller than the size of the enzymes can be used so that not only is leaching prevented but also the selection of enzymes and MOFs is greatly expanded; therefore, researchers could use a wider range of MOFs to impart various interesting functions to different enzymes. Furthermore, an enzyme maintains its biological function under a wider range of conditions after being embedded in metal−organic framework (MOF) microcrystals via this de novo approach. This enhanced stability arises from confinement of the enzyme molecules in the mesoporous cavities in the MOFs, which reduces the structural mobility of enzyme molecules. A fluorescence spectroscopy study shows that the structural conformation of the embedded enzyme changes less under these denaturing conditions than free enzyme.

    Introduction of Lecturer:


    2002-2007        Ph.D. in Chemistry/Biochemistry, University of California at Santa Barbara (UCSB), Advisor: Professor Norbert Reich. February 2007

    1994-1996        M.S. in Bio-Analytical Chemistry, National Chiao-Tung University, Advisor: Professor Allen C. Chang (國立交通大學生科所 張正教授)

    1990-1994        B.S. in Biochemistry from the Department of Applied Chemistry, National Chiao-Tung University, Taiwan (國立交通大學應用化學系)


    2014-                 Associate Professor in Chemistry, National Central University,

    2008-2014        Assistant Professor in Chemistry, National Central University

    2007-2008        Postdoctoral Research Associate in Chemistry, University of California at Los Angeles


    2-year MOST Grant for Excellent Young Scholar, Aug. 2016 - July 2018

    The scholarship of government sponsorship for overseas study 1999

    Selected Publications

    1.                Fa-Kuen Shieh* et al. "Shielding Against Unfolding by Embedding Enzymes in Metal-Organic Frameworks via a de novo Approach" J. Am. Chem. Soc. (2017) 6530-6533.

    2.                Fa-Kuen Shieh* et al. "Green and Rapid Synthesis of Zirconium Metal-organic Frameworks via Mechanochemistry: UiO-66 Analog nanocrystals obtained in one hundred seconds" Chem. Comm. 2017, 53, 5818-5821.

    3.                Fa-Kuen Shieh* et al. "Cytotoxicity of Postmodified Zeolitic-Imidazolate Framework-90 (ZIF-90) Nanocrystals: Correlation between Functionality and Toxicity" Chem. Eur. J.  9 (2016) 2925-2929.

    4.                Fa-Kuen Shieh* et al. "Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de novo Approach: Size-Selective Sheltering of Catalase in Metal-Organic Framework Microcrystals" J. Am. Chem. Soc. (2015) 137 (13), 42764279.

    5.                F.-K. Shieh* et al. "Synthesis of hierarchical micro-/mesoporous structures via solid-aqueous interface growth: zeolitic imidazolate framework-8 (ZIF-8) on siliceous mesocellular foams (MCF) for enhanced pervaporation of water/ethanol mixtures". ACS Appl. Mater. Interfaces (2014), 6 (7), 5192–5198.

    6.                F.-K. Shieh* et al. "Water-Based Synthesis of Zeolitic Imidazolate Framework-90 (ZIF-90) with a Controllable Particle Size" Chem. Eur. J. 34 (2013) 11139 –11142 (Selected as Back Cover).

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